Rise Against at Red Hat Amphitheater

It was a perfect day at Red Hat. Sunny and a great, happy, excited crowd. Good to see. Multiple bands were playing, and although I’ve had to pause shooting shows for a while to stay home to care for my 90 year old mom, I was asked if I wanted to shoot Rise Against, and well…I just couldn’t say no!
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I used the Cannon 5D Mark II Bodies with the 70-200mm 2.8, 16-35mm 2.8, and the 14mm 2.8 Canon lenses.
I see I was using a 320 shutter speed, ISO 640 at F3.2, or there about.
_MG_0173You could say that all of us photographers had to deal with lots of photographers with twice as many elbows. But I did get to see a few old timers there and it’s always enjoyable to reconnect in this odd reality called the photo pit. All things being turned on, tuned up and tuned in, and always with these giant security dudes ready to bust your ass if you go over the line. YES SIR!
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_MG_0062I was asked to cover the crowd a bit which I seldom do as it most often interferes with venue rules. But with my trusty set of instructions from the band’s agent I was allowed just a few minutes to go out and about on the grounds. I could stay all day out among the savages…I guess that’s what tickets are for.

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    The C/Si/Ge analogues are derivatives of the GnRH antagonist, which bears an -tyrosine residue [instead of the -Me3C-Ala, -Me3Si-Ala, or Me3Ge-Ala residue] in position 5 of its decapeptide backbone.
    All compounds behaved as potent GnRH antagonists , the binding affinities and antagonistic potencies of the
    three C/Si/Ge analogues being fairly comparable. Compounds had been additionally studied for their in vivo
    actions in male rats after s.c. They produced both a strong testosterone suppression (single-dose remedy,
    1.5 mg/kg) and a powerful LH suppression (castrated male rat; singledose treatment, 0.05 mg/kg).

    78 In the synthetic approaches to the polyketide section 3-0TBS-7-OPMB-2, 5, eight,
    8-tetramethylnonanoic acid, key steps concerned asymmetric allylation, crotylation, and asymmetric conjugate additions.

    eighty Both strategies are very environment friendly and proceed with high
    ranges of stereocontrol throughout. New cytochalasans, penochalasins D-H 15-19, had
    been isolated from a pressure of Penicillium sp.ts originally
    separated from the marine alga Ebteromorpha intestinealis, and their
    stereostructures had been established primarily based on spectral information. All compounds exhibited important cytotoxic activity (ED so 3.2, 2.1, 1.8,
    1.9, 2.8 Ilg mL-\ respectively) in opposition to cultured P388 cells.
    A protein-bound pigment, allophycocyanin, purified from the blue-inexperienced alga Spirulina platensis, is the primary compound reported to exhibit anti-enterovirus seventy one activity .
    Enterovirus seventy one an infection causes vital morbidity and mortality in children,
    but there isn’t a efficient therapy.

    Maintenance Of Chromatin States And Carcinogenesis

    Topoisomerase II is involved in DNA unwinding throughout replication and transcription and in addition participates in genetic
    recombination, chromosome condensation, and apoptosis .
    TRANSCRIPTIONAL REPRESSION MEDIATED BY HDAC1 HDAC1 Substrate Specificity HDACs are believed to repress transcription mainly
    by way of deacetylation of the histone tails that protrude from
    the nucleosomes, leading to local modification of chromatin structure .

    HDAC1 has been shown to deacetylate all four core histones in vitro and seems to preferentially deacetylate specific lysine residues on histone
    H4 . The enzyme has additionally been discovered to deacetylate a subset of histones H3 and H4 in vivo .
    In addition to histones, HDAC1 can even deacetylate
    nonhistone proteins, such because the tumor suppressor p53 , the transcription factors
    E2F1 and YY1 , and proliferating cell nuclear
    antigen . Selenium compounds similar to seventy seven, ebselen or selenodiglutathione , are chemopreventive in animal fashions,
    inhibit cell growth and induce apoptosis in vitro. Compound 77 has been probably the most extensively investigated.

    With higher doses these results have been masked or coated
    by different, extra pronounced and quicker creating deadly effects of
    the toxin, such as platelet aggregation. It is subsequently affordable to assume that
    the AChEinhibitory results so apparent in in vitro experiments usually are not answerable for the deadly activity of the toxin.
    Lyngbyabellin E 128, at concentrations of zero.01-6.zero jlM,
    disrupted the mobile microfilament network in A-I zero cells.

    exhibited potent cyto toxicity in vitro with IC
    so values of 0.fifty two nM in opposition to KB and 0.36 nM towards LoVo cancer cells; however, it was only marginally active
    in vivo towards a colon tumor and ineffective towards a mammary tumor.

    Syntheses of apratoxin A, its analogue and segments have been reported.
    The complete synthesis of 103 featured stereocontrolled entry to the novel polyketide domain and the late-stage installation of the delicate 2,4-disubstituted
    thiazoline moiety using Staudinger seventy six reduction-intramolecular aza-Wittig process.
    In the synthesis of an oxazoline analogue of apratoxin A, the success
    in macrocyclization on the N-Me-lle-Pro web site demonstrated again that this web site is an appropriate macrolactamization site for synthesizing
    apratoxins and their analogues.
    It inhibits cell proliferation and induces apoptosis in a number of tumor cells.

    Ebselen supplies an environment friendly safety towards mustard-induced cell dying
    in normal and tumoral lymphocytes and would possibly show useful as
    an antidote towards alkylating agents. Compound 103 is associated with induction of Fas ligand,
    a well known mediator of apoptosis, and activation of so-known as
    stress kinase signaling pathways, significantly in Jun NH2 -terminal kinase.

    Their experiments showed a rapid blockade of motion potentials conduction, with a slow
    and partial depolarization. The whole blockade of conduction was only partially reversible and with out full recovery of the management spike and resting potential.
    In principle, this activity may be linked to the non-particular action of halitoxin towards the cell membranes, which prevents nerve conduction and kills the experimental
    animal by respiratory paralysis, as already reported by Kaul
    and Daftari . Berlinck et al. additionally argued that the in vitro
    and in vivo cytotoxic impact reported for the crude sponge extract containing halitoxin is
    quite a consequence of the toxin antiproliferative or antimitotic activity than as
    a result of its cytolytic exercise.
    the conformation of artificial compound was near that of
    the palau’amide, however some stereochemistry
    within the structure of the palau’amide was incorrectly assigned.
    91 Five new lyngbyabellin analogs lyngbyabellins E-I have been isolated from the marine
    cyanobacterium Lyngbya majuscula collected from Papua New Guinea.

    ninety two All 5 lyngbyabellins confirmed cytotoxicity to NCI-H460 human lung
    tumor and neuro-2a mouse neuroblastoma cell traces with LC so values between 0.2 and four.8 jlM.

    The anti-proliferative impact of HDAC inhibitors is in fact the premise for medical studies of those molecules in anti-cancer therapies .

    The acetylation state of histone is reversibly regulated by histone acetyltransferase and deacetylase
    .

    Accordingly, HDAC inhibitors could also be explored as therapeutic agents for the treatment of ADPKD.

    Recently, these key enzymes in the gene expression had been cloned.

    For the silicon- and germanium-containing decapeptides forty two and 43 the testosterone and LH
    suppression lasted for a considerably longer time frame in contrast with the consequences of the carbon analogue forty one.

    different hand, the lethal motion of halitoxin could possibly be also linked to its neurotoxic exercise, observed by the same
    authors on isolated crustacean nerve preparation.

    Biologically Active Compounds From The Household Mycalidae

    In vitro, poly-APS induced thrombocyte aggregation in a dose-dependent manner.
    The mechanism of aggregation was not explained but attributed to the potential non-specific
    action in direction of endothelial cells. Altogether,
    the AChE-inhibitory effects were pronounced solely
    at decrease doses of polyAPS.
    Two strains had been isolated from the water column, two from the close by rocky substrate and
    one from the sediment. The sponge extracts
    had been examined at natural concentrations and haliclamines C and D
    have been examined at 5 mg/mL. Haliclamines showed a robust inhibition against Polaribacter and
    very sturdy exercise towards Planococcus. Some alkylpyridinium compounds also show antifungal exercise.

    Xestamines A-F (19-24) from Calyx podatypa were energetic towards Candida albicans .
    Haliclamines A and B have been additionally reported to own antifungal exercise against Mortierella ramannianus .

    In order to gauge the position of AChE inhibition in toxin lethality,
    and to evaluate different attainable lethal results, in vivo experiments have been performed on male Wistar rats,
    and electrocardiogram , blood pressure and respiration pattern were
    monitored. The outcomes confirmed that not one of the animals
    died due to the AChE-inhibitory action ofpoly-APS. Doses decrease than Img/kg triggered only transient bradycardia and transient prolongation of expirium.
    At doses above 2.7 mg/kg of poly-APS, all handled animals died,
    however signs were not typical of AChE inhibition. Arterial blood strain fell to mid-circulatory pressure, and respiratory
    stopped after a few breaths, with an increase of the residual quantity.
    Autopsy of the experimental animals that died because of the
    effects of the toxin revealed that mid-dimension and small sized blood vessels in the coronary heart and lungs had been full of granular brownish material with inclusions of pink blood cells and platelets.

    Data obtained on blood samples from animals treated with polyAPS also revealed quite a few thrombocyte aggregates.

    Histone Acetylation, Protein Acetylation, And Gene Regulation

    Allophycocyanin neutralized the enterovirus seventy one-induced CPE in each human rhabdomyosarcoma cells and African green monkey kidney cells.
    caprolactans with antiviral exercise in the direction of
    HSV-2 have been isolated from one other marine bacterium .
    As we can see above, marine microorganisms represent an underexplored resource for the invention of novel antiviral brokers.

    Rowleyet al. described a series of peptides designated halovirs A-E, Fig.
    which are produced from a marine-derived fungus of the genus Scytalidium.

    These lipophilic and linear peptides are potent in vitro inhibitors of
    HSV1 and HSV-2.

    Isolation and Characterization of Triterpenoids from Laurencia Thyrsiferol [1, Fig. ] was isolated from the pink
    algae Laurencia thyrsifera that was collected
    off the coast of New Zealand. It constitutes the first example of a triterpenoid squalene-derived polyether of
    marine origin containing a dioxabicyclo[four.4.0]decane B-C framework.
    The unique framework of thyrsiferol consists of a central trans-fused pyranopyran unit, an appended cyclic bromo
    ether and an aliphatic aspect chain connecting the central unit to
    a transtetrahydrofuran ring. Isolation and spectroscopic characterization of thyrsiferol was first carried
    out by Munro et al in 1978 . An x-ray crystallographic analysis of its C l8 -acetate
    derivative established its chemical construction and assigned every of the relative stereocenters.

    Kelman and associates have assessed the antimicrobial activity of the halitoxinlamphitoxin combination that
    was isolated from the Red Sea marine sponge Amphimedon viridis.
    The combination was examined in opposition to ecologically related micro organism
    and resulted in zero activity against spongeassociated micro organism, but robust inhibition was found
    towards most strains isolated from seawater. This selective inhibition might play an necessary
    ecological position in preventing microfouling and
    in defence towards potentially pathogenic marine bacteria.

    A similar examine was carried out with haliclamines (49-52) isolated from the Arctic sponge Haliclona viscosa .
    The extracts and pure compounds from this sponge have been examined towards 5 bacterial strains from pure habitat of the sponge.

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